Kinetics of hyperosmotically - stimulated Na - K - 2 Cl cotransporter 8 in Xenopus laevis oocytes
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1 2 3 4 5 6 7 Kinetics of hyperosmotically-stimulated Na-K-2Cl cotransporter 8 in Xenopus laevis oocytes 9 10 11 12 Eric Delpire and Kenneth B. Gagnon 13 Department of Anesthesiology, Vanderbilt University School of Medicine, 14 Nashville, Tennessee 37232 15 16 17 18 Running title: Na-K-2Cl cotransporter kinetics in frog oocytes. 19 20 21 22 23 24 Address correspondence to: 25 Eric Delpire, 26 Department of Anesthesiology, 27 Vanderbilt University Medical Center, 28 T-4202 Medical Center North, 29 1161 21st Ave. South, Nashville, 30 TN, 37232-2520. 31 Tel: 615 343-7409. 32 Fax: 615 343-3916. 33 E-mail: [email protected] 34 35 36 37 38 39 40 41 Articles in PresS. Am J Physiol Cell Physiol (July 20, 2011). doi:10.1152/ajpcell.00131.2011
منابع مشابه
Kinetics of hyperosmotically stimulated Na-K-2Cl cotransporter in Xenopus laevis oocytes.
A detailed study of hypertonically stimulated Na-K-2Cl cotransport (NKCC1) in Xenopus laevis oocytes was carried out to better understand the 1 K(+):1 Cl(-) stoichiometry of transport that was previously observed. In this study, we derived the velocity equations for K(+) influx under both rapid equilibrium assumptions and combined equilibrium and steady-state assumptions and demonstrate that th...
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The K-Cl cotransporters (KCCs) have a broad range of physiological roles, in a number of cells and species. We report here that Xenopus laevis oocytes express a K-Cl cotransporter with significant functional and molecular similarity to mammalian KCCs. Under isotonic conditions, defolliculated oocytes exhibit a Cl(-)-dependent (86)Rb(+) uptake mechanism after activation by the cysteine-reactive ...
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تاریخ انتشار 2011